The molar ratio of Mo(SCN)6-, OOBAR+ was 1:1 in acidic medium. The equilibrium isotherms, kinetics and thermodynamic models for Mo(V) sorption onto OOBAR were studied. Thermodynamic parameters such as enthalpy (?H), entropy (?S) and Gibbs free energy (?G) were -46.2 kJ/mol, -0.082 kJ/Kmol and -21.8 kJ/mol was shown that the sorption process was spontaneous, exothermic nature with decrease disorder and randomness at the solid-liquid interface of Mo with OOBAR. Dynamic experiments using glass column was indicated a good affinity chromatographic separation for its applications in many pharmaceutical and biological samples including liver mice tissue and pharmaceutical vitamin drugs. The lower relative standard deviation (RSD%) value for pharmaceutical applications samples (n=5) was found from 1.1%.